The production of polypeptides is generally divided into chemical synthesis and biosynthesis.
1. chemical synthesis: chemical synthesis is a common polypeptide production method, which constructs polypeptide chains by gradually connecting amino acid units through organic synthesis reactions. This method can produce polypeptides of various lengths and sequences. The steps of chemical synthesis include:
-Select appropriate protective base and activating reagent;
-;
-The target peptide was finally obtained by deprotection and purification.
2. biosynthesis: biosynthesis is the use of biosynthetic mechanisms in organisms to produce polypeptides. One of the common methods is to use genetic engineering technology to express target peptides in bacteria or yeast such as Escherichia coli. The steps include:
-The gene sequence of the target polypeptide was designed and inserted into the appropriate expression vector;
-Transformed bacteria or yeast cells, cultured and induced expression;
-The expression product was harvested, and the pure polypeptide was obtained by purification and other processing steps.
Whether chemical synthesis or biosynthesis, the production of polypeptides requires strict control of reaction conditions, purification steps and quality detection to ensure the purity and quality of polypeptides. . In practice, it is necessary to select a suitable method to produce peptides according to the specific situation.
